① embedded with a hard wax embedded in general, that the melting point of paraffin wax 60-62 ℃. Consistent paraffin-embedded with a melting point of the melting point of paraffin and wax dipped to use. Paraffin wax dipped slightly higher than the temperature of the drug embedded with temperature ② maintained at 65-68 ℃, but not too high, otherwise easily cause burns tissue, and experimental results. ③ embedded operation to quickly, so as not to block the organization has not been buried properly before melting wax solidifies. ④ mucosa, wall, skin tissue must be perpendicular to the ground embedding cassettes, commonly called Li buried or stand package. Vessel wall should stand buried. ① sharp knife to slice, cut no defect, Jiangsu section, in order to cut out the entire sheet. At present, many with a disposable blade slices, slice of good quality. If using heavy duty all steel, it has often ground to maintain its sharp, no defects. ② bone and other calcified tissues must be stripped of calcium, parasitology section, and then said later steps of the process, in order to slice. 2. Rinse: The material removed since the fixative, rinse immediately required to make all of the fixative among all removed to prevent the stain some fixative, such as 10% formalin, Bouin's solution fixed material, if time shorter words need not be washed. But also need to wash too long 3, dehydration (37 degrees incubator) is the use of so-called dehydrated dehydrating agent will displace the water within the organization, human histological sections to facilitate the infiltration of organic solvents, a process called dehydration. Dehydration is complete, is directly related to whether the organization can fully transparent excessive dehydration (due to tissue in pure alcohol it takes too long, tissue texture changes occur) is likely to cause tissue brittle. The cause of the organization as well as crisp heating (the temperature exceeds 36 ℃), within a dehydrating agent added acetone, xylene and excessive use of dipped wax paraffin and the like. Generally, we always believe organizations crisp with a high concentration of alcohol-related, biological sliced ??manufacturers, while ignoring the relationship between low levels of alcohol, in fact, a low concentration of alcohol has a strong penetration, easier penetration into the tissue than pure alcohol inside the block to make The dehydration, tissue if sufficiently dehydrated at low concentrations of alcohol, in the high concentration of alcohol in simply removing moisture from a low concentration to a high concentration between, therefore, can be completed in just a short time, if the time is not shortened pure Alcoholic time, it will cause tissue crisp; if dehydration heating, using acetone, can cause tissue contraction and affect immunohistochemical markers zoology sections voyage teaching (certified), Jiangsu sliced ??by the Xinxiang City. Teaching Equipment Co., Ltd. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is a leader in Xinxiang specimen model years, the company implement the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.