Precipitation tissue fixation, to remove tissue infiltration and fixative fixative produced, crystallization, coloring matter, otherwise it will affect the future of dyeing effect, the process is fixed after treatment. Processing method according to the fixative to choose, microbiology sliced ??through neutral paraformaldehyde-fixed tissues generally use running water for 24 hours to process (prior to running water, the tissue can block proper trim, easy embedding and sectioning of production). In addition, calcium-containing tissues, such as: bones, teeth, etc., should be carried out after rinsing decalcification. General use of calcium EDTA organization utterly decalcification, this method of tissue without damage, decalcified slow activity retention, less damage to tissue antigens. Embedding: embedding refers to the organization after the package Baptist wax process is a piece with embedding medium (paraffin, collodion, resins, etc.). Tissue bag method most commonly used is paraffin embedding, made of wax blocks. Slice: Slice refers slicer paraffin tissue blocks depending on the thickness of the sheet and cut into tissue test requirements. Slide off the backing sheet to prevent human histological sections, the tissue sections firmly attached to the top of the slide. Commonly used rendering method is polylysine backing. Test equipment and test reagents: 1. Equipment: surgical instruments, laboratory animals, jars, fixative, label sheet 2, fixed: formaldehyde fixative fixative: 1 part liquid formaldehyde +9 parts of water, Guiyang slice, dubbed proportion 4% formaldehyde, or 10% formalin. 4% neutral formalin configuration: 40% formaldehyde 100ml, distilled water 900ml, botanical sections, sodium dihydrogen phosphate (? NaH2PO4 H20) 4g, disodium hydrogen phosphate (anhydrous) 13g, a pH value of 7.0 ethanol fixative. : for a fixed concentration of typically 80% -95%. 3, dehydration: Leica dehydrator, 70%, 80%, 90%, 95% ethanol. 4. Transparency: xylene (benzene, toluene, chloroform, cyclohexanone, etc.) 5, the Baptist wax: Leica embedding machine, soft wax (melting point 54 ℃ or less), hard wax (melting point 60-62 ℃). 6. Slice: Leica slicing machines, disposable blades, poly-lysine, slides, water bath, oven. Guiyang slice | voyage teaching | Human histological sections provided by the Xinxiang City Teaching Equipment Co. voyage. Guiyang slice | voyage teaching | Human histological sections Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) this year introduced a new upgrade, the above picture is for reference only, please dial this page Phone or pictures on obtain the latest information, Contact: Manager Ma.