However, exports of bio-chips, paraffin-embedded tissue DNA content by flow cytometry analysis of paraffin-embedded tissue sections used in conjunction with flow cytometry to measure the DNA content and ploidy analysis, this combination is flow cytometry in clinical application in particular, we are opening up new avenues of research in cancer research. Now measurable cell size, volume, DNA content, DNA synthesis rate, RNA content, surface antigen, chromosomes. Because of sample preparation techniques, past many data flow analysis is limited to the use of fresh tissue samples, but can now analyze paraffin-embedded tissue sections prepared dispersed cell suspension technique to detect DNA content can be obtained from a sufficient number of tissue slices individual cells, and a single cell with fresh tissue isolated on morphology and DNA content of the group were very similar Square. General steps we all know, do not say 1. drawn: organization as fresh as possible, the sooner the better quench, high biological chips, in order to avoid ice. The ice crystals will produce irreparable cell damage, directly affecting results of immunohistochemistry. Therefore, the general will be the critical surface tissue suspended in liquid nitrogen for 10-20 seconds organization quenched in liquid nitrogen. 2. The cryostat temperature is usually in the freezer for 10 minutes, cooled to near room temperature and then cut, usually organized in -15 ~ -20 ℃ sections most likely to succeed. Each organization has its appropriate slice temperature, knife temperature and cold room temperature. 3. Because sliced ??fresh frozen tissue sections and quench embedded in OCT, biological microscope sections, in fact, the organization itself does not penetrate into the embedding agent, so the organization itself is still relatively soft. The sections of this relatively high technical requirements, requiring sharp blade, blade angle well, tissue temperature and chip temperature and the temperature difference between the appropriate box; when requested slice feed speed and strength to be a good grasp to cut out a satisfactory of the film. The leading edge of the anti-Coil flank shall be sufficient distance so smooth slice through. Anti-coil flank is slightly higher than the highest point, was empirically adjust the knife angle to the organization. However, paraffin-embedded tissue DNA content by flow cytometry analysis of paraffin-embedded tissue sections used in conjunction with flow cytometry to measure the DNA content and ploidy analysis, this combination is flow cytometry in clinical applications, biological chips , especially in cancer research has opened up new avenues of research. Now measurable cell size, volume, DNA content, DNA synthesis rate, RNA content, surface antigen, chromosomes. Because of sample preparation techniques, past many data flow analysis is limited to the use of fresh tissue samples, but can now analyze paraffin-embedded tissue sections prepared dispersed cell suspension technique to detect DNA content can be obtained from a sufficient number of tissue slices individual cells, and a single cell with fresh tissue isolated on morphology and DNA content of the group were very similar Square. Biological slice voyage teaching (certified), biological chips provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is a leader in Xinxiang specimen model years, the company implement the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.