However, paraffin-embedded tissue DNA content by flow cytometry analysis of paraffin-embedded tissue sections used in conjunction with flow cytometry to measure the DNA content and ploidy analysis, this combination is flow cytometry in clinical applications, especially in cancer research has opened up new avenues of research. Now measurable cell size, volume, DNA content, DNA synthesis rate, RNA content, surface antigen, chromosomes. Because of sample preparation techniques, past many data flow analysis is limited to the use of fresh tissue samples, but can now analyze paraffin-embedded tissue sections prepared dispersed cell suspension technique to detect DNA content can be obtained from a sufficient number of tissue slices individual cells, and a single cell with fresh tissue isolated on morphology and DNA content of the group were very similar Square. 2. To prevent dehydration excessive dehydration primers tissue atrophy. If not timely dehydration levels, the material can be placed in 70% alcohol in preservation, quality bio-chips, because of the high concentration of alcohol is easy to shrink the tissue hardening process should not be too long. 3. The immersion time transparent agent will have The tissue material block size and belong to the substance of the organ cavity Or may be. If the time is too short and transparent, bio-chips, the transparency is not complete, it is difficult dipped in paraffin tissue; transparent too long, the tissue hardening brittle, it is not easy to cut out the entire section, up to several hours. The slice thickness, slice too thin and too thick will affect the next experiment, the patch is too thin to do well with grilled slices, staining results may not all; too thick, it may be difficult to see staining, or through through a lot of trouble when. 5. antigen retrieval, the biggest bad place paraffin is to antigen retrieval, a fix is ??not good, not the best immunostaining beautiful, this is a lot of people choose to frozen sections biggest reason, because there may be one of your Repair optimized antigen will cost you a lot of time here. 2. To prevent dehydration excessive dehydration primers tissue atrophy. If not timely dehydration levels, the material can be placed in 70% alcohol in preservation, due to the high concentration of alcohol is easy to shrink the tissue hardening process should not be too long. 3. The immersion time transparent agent will have The tissue material block size and belong to the substance of the organ cavity Or may be. If the time is too short and transparent, bio-chips, the transparency is not complete, it is difficult dipped in paraffin tissue; transparent too long, the tissue hardening brittle, it is not easy to cut out the entire section, up to several hours. The slice thickness, slice too thin and too thick will affect the next experiment, the patch is too thin to do well with grilled slices, staining results may not all; too thick, it may be difficult to see staining, or through through a lot of trouble when. 5. antigen retrieval, the biggest bad place paraffin is to antigen retrieval, a fix is ??not good, not the best immunostaining beautiful, this is a lot of people choose to frozen sections biggest reason, because there may be one of your Repair optimized antigen will cost you a lot of time here. _ _ Voyage supply biological slice sliced ??biological teaching provided by Xinxiang City Teaching Equipment Co. voyage. _ _ Sliced ??biological organisms slices teaching voyage supply Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) this year introduced a new upgrade, the above picture is for reference only, please call this page or image Contact phone, for the latest information, Contact: Manager Ma.