The following briefly describes several aspects from the principle of common staining technique: a display of succinate dehydrogenase (SDH) activity nitro -BT law: (Succinate dehydrogenase) ⑴ positioning enzymes and biological significance: succinic off mitochondrial marker enzyme catalase, which is present in all cells in tissue sections of aerobic respiration processes, and in close connection with the mitochondrial inner membrane. Without coenzyme and enzyme itself has flavoprotein (FP, Flavoprotein) supplemented groups. In the group of reaction is often used to reflect the situation in the citric acid cycle. ⑵ Principle: The final step of the above reaction principle is tissue slices manufacturers, nitro -BT (Nitro-blue tetrozolyum, tetrazolium) systems can vary tetrazolium ring compounds, when it accepts the hydrogen itself is reduced to a colored The precipitated product (A) (Formazan). Slice preparation routine operating procedures and requirements (i) The sequence block associations were: ① washing, ② dehydrated animal tissue sections, ③ transparent, ④ dip wax, ⑤ ⑥ embedding and sectioning. (Ii) Preparation and HE communities sliced ??dyeing process using ethanol, acetone, xylene, paraffin and other flammable, toxic, have someone manage tissue sections, 2m or less do not have to suffer from a flaming fire, the local environment should be good The ventilation and fire-fighting facilities. (Iii) regular slice manual (step sequence and duration of each step) 1. Wash: Rinse with water associations have fixed block 30min. 2. Dried (room temperature) (1) ethanol - formaldehyde (AF) were fixed 60 ~ 120min (2) 80% ethanol, 60 ~ 120min (3) 95% ethanol Ⅰ 60 ~ 120min (4) 95% ethanol Ⅱ 60 ~ 120min (5 ) ethanol Ⅰ 60 ~ 120min (6) ethanol Ⅱ 60 ~ 120min (7) ethanol Ⅲ 60min [Note] ① inadequately fixed into the community does not suffer from dehydration process. ② for dehydration reagent volume should be at least 5 to 10 times the total volume of the block association. ③ Since the low concentration of ethanol to progressively move into high concentrations of ethanol dehydration. ④ dehydrating reagents should promptly filter replacement (500ml ethanol can be used in 500 communities block dehydration; ethanol turns blue when adding copper sulfate, suggesting that needs to be replaced). ⑤ larger community block longer than the lesser of dehydration, dehydration should separate the two. ⑥ societies for the time not too long inside ethanol (to avoid hardening). ⑦ acetone dehydration strong performance, block associations will shrink too, hard and brittle, not to replace ethanol. Tissue sections produced tissue sections _ _ Changsha voyage voyage teaching provided by Xinxiang City Teaching Equipment Co., Ltd .. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future! You can also access (www.yuanhang369.com/supply_details_41826990.html), for more information about the company.