Transparent (1) Xylene Ⅰ 20min (2) xylene Ⅱ 20min (3) Xylene Ⅲ 20min [Note] ① volume xylene should be 5 to 10 times more than the total volume of the block association. ② Societies transparent block in xylene time not too long (to prevent community hard, brittle), and depending on its size and different categories of society; associations appear transparent to brown or dark red. ③ xylene should be filtered and replaced. ④ When societies after appropriate treatment is not significant degree xylene transparent, often suggesting that the association is not fully fixed or dehydration, you should find out the reasons and appropriateness of treatment. Baptist wax (a) paraffin Ⅰ (45 ~ 50 ℃) 60min (2) paraffin Ⅱ (56 ~ 58 ℃) 60min (3) paraffin Ⅲ (56 ~ 58 ℃) 60min [Note] ① melted paraffin must have the person responsible, must be in the melting wax box or bath (70 ℃) were not suffering from a flaming fire by heating. ② melting wax volume should be at least 5 to 10 times the total volume of the block associations. ③ communities block by xylene degree only after proper transparent wax dipped into the process, should be as clear cut after xylene community block surface into molten wax. ④ appropriate time Baptist wax, wax dipped too short inadequate (community too soft), when long association brittle. ⑤ melting wax should be filtered and replaced. ① ethanol dehydration dehydration levels above time can be adjusted according to the size of the organization blocks. For small tissue blocks (such as the gastric mucosa, bronchial mucosa, etc.) may be appropriate to reduce the drying time, for large tissue mass can be increased drying time, but do not exceed the maximum levels of ethanol supply 12h tissue sections, not more than ethanol 4h. If unable to continue in the middle, you can return the specimen to save 80% ethanol. Further, adipose tissue and loose connective tissue in general should be extended the dehydration time, in 95% ethanol, water must be off the net, the fat must be dissolved away (ethanol concentration of 50% or more fat soluble and lipids). Otherwise, paraffin can not penetrate the fat cells and fibrous tissue, can not cut out the good slice, but also easy to off-chip staining. ② If the tissue contained too much water (such as embryonic tissue), the dehydration of ethanol concentration should be lower-level (eg 30-40% ethanol) began tissue sections, which is to ensure the production quality is very important. ③ If dehydration different tissue sections suppliers, slice after slice wax block tissue often appears depressed (because of wax-embedded tissue blocks in contact with air and drying shrinkage). Such a wax block, though barely able to cut out sections, but the effect of poor pathological staining tissue sections stained tissue can easily fall off. Therefore, you must complete dehydration, dehydration or not is completely good or bad quality of an important part of the producer. ④ volume dehydrating agent should be tissue blocks 5-10 times the total volume of dehydrating agents should be filtered and replaced. Tissue sections Supply | voyage teaching (certified) | Fuzhou tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_42264970.html), for more information about the company.