1, fixed with a tissue protein coagulation or precipitation effect, so can inhibit the activity of enzymes and bacteria breeding glycolysis, thus rendering the fixed effects (1) 10% formalin solution for organizations: 1 + 9 formaldehyde solution the parts of distilled water marketed formaldehyde solution containing about 37-40% formaldehyde, 10% formalin solution is actually formaldehyde-only range of 3.7-4.0 percent for: a variety of organizations. However, in 10% formalin fixed tissue, nuclear staining better, and cytoplasmic staining of poor treatment: Organization blocks generally fixed for more than 24h, for a long time can even be used to block the long-term preservation organization. Short-term fixed tissue blocks without washing, can be directly transferred to 70% alcohol dehydration (2) Bouin's solution: saturated picric acid solution (1.22%) 75ml 25ml formaldehyde, acetic acid solution 5ml Scope: a variety of animal tissues and plant tissues apical, animal tissue fixative good deal: 24h fixed tissue sections process can also be saved in this long-term solution. Without running water or washed directly into the 70% ethanol dehydration. ① xylene transparent volume should be more than the total volume of tissue blocks 5-10 times. ② transparent tissue mass in xylene time should be adjusted appropriately according to the size and freshness liquid tissue types and tissue blocks, such as brain tissue and blood clots to be shorter retention time in xylene, and muscle tissue should be extended retention time in xylene. ③ If tissue blocks when entering xylene still significant when cloudy or clear after tissue blocks processed through xylene modest, indicating that the organization is not fully dehydrated, should find out the reasons. ④ xylene should be filtered and replaced. ① Baptist Baptist wax wax thermostat to master the appropriate tissue slices manufacturers, the temperature is generally higher than the melting point of paraffin to 2-3 ℃, generally maintained at 65 ℃ appropriate. If the temperature is low, paraffin began agglutination, then reach Baptist wax purpose, the temperature is too high, dipping wax hardened tissue often contracted tissue sections, while animal tissue sections, tissue antigens susceptible to damage, thus affecting Immunohistochemical staining of enzyme markers. ② time can be used for dipping wax block size of the organization appropriately adjusted according to a variety of small biopsy specimens and tissue mass animal specimens taken generally small, the above should be relatively short time dipping wax, wax dipped in 1h total time is appropriate. ③ melting wax volume should be 5-10 times more than the total volume of tissue blocks. ④ should minimize the rear surface of the transparent tissue blocks into xylene melting wax. ⑤ soaked with paraffin wax should be replaced regularly to ensure purity, exchange about once a month or so. Tissue sections flow _ _ voyage teaching Xiangfan tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future! You can also access (www.yuanhang369.com/supply_details_44276095.html), for more information about the company.