2. Rinse: Since the fixative material removed, rinse immediately need to make all of the fixative which all removed to prevent the dyeing some fixative, such as 10% formalin, Bouin's solution fixed material, if time shorter words need not be washed. But also need to wash too long 3, dehydration (37 degrees thermostat) is the use of so-called dehydration dehydrating agent will displace the water within the organization to facilitate the infiltration of organic solvents, a process called dehydration. Dehydration is complete, is directly related to whether the organization can fully transparent excessive dehydration (due to the organization of pure alcohol in a long time, the occurrence of tissue texture change) is likely to cause tissue brittle. The cause of the organization as well as crisp warm (temperature exceeds 36 ℃), within the dehydrating agent added acetone, xylene excessive use wax dipped in paraffin and so on. Generally, we always think crisp organization concerned with the high concentration of alcohol, while ignoring the relationship between low levels of alcohol, in fact, the low concentration of alcohol has strong penetration and easier than pure alcohol to penetrate into tissue inside the block to make the dehydration, the organization If sufficiently dehydrated alcohol in a low concentration, the high concentration of alcohol in simply removing water from a low concentration to a high concentration of between, therefore, only a short time can be completed, if the time does not shorten the time of pure alcohol, will cause tissue brittle; if dehydration heating, acetone, can cause tissue contraction and affect immunohistochemical markers (7) to adjust the slice thickness regulator (usually 4 ~ 6μm), slice, cut. out of the wax into the band should be continuous, complete integrity, appropriate thickness (3 ~ 5μm), uniform tissue sections produced no marks, chatter marks, wrinkles, cracks, defects, release and so on. (8) with special tweezers to gently gripping complete, no marks, uniform thickness of wax, stretching into the warm water (about 45 ℃), so slice in full swing. [Note: The water temperature must be affordable, clean (especially water); finish after each cut a wax block tissue slices processes must be carefully clean up the water, do not suffer from the debris left over from societies other cases of animal tissue sections, in order to avoid contamination. ] (9) The wax to be affixed or coated with protein by glycerol 3-aminopropyl - triethoxysilane (3-aminopropyltriethoxy silane, APES) treated glass slides (HE staining when using, where appropriate, may be omitted , if necessary,). Wax tablets should be placed in a glass slide right (or left) of the Central Organization Department sliced ??2/3, leaving slides left (or right) position for 1/3 label attached. No air bubbles between the wax and slides. 10) must be placed immediately at the end of the wax slides (to be attached to one end of the label), with a number of high quality engraved pen or marker accurately, clearly marking the corresponding pathology number (including sub-number). [Note: You must ensure that exactly the same number of pathology associated with the pathology community numbers paraffin blocks on the slide, do not suffer from leakage write pathology write or wrong number. ] (11) The placement of the wax slide oblique angle was 45 ℃ moment; the water to be shed on the rear slides, placed in the oven baking (60 ~ 62 ℃, 30 ~ 60min), You can then stained. Histopathologic sections _ voyage teaching (certified) _ Yangquan tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_45796400.html), for more information about the company.