4, transparent (56-60 degrees thermostat) in order to make paraffin dipped into the tissue blocks, you must go through a mixing both with a dehydrating agent, but also media compatibility with paraffin material, this process is called transparent. Currently the best transparent agent is xylene, at room temperature, if not too long (more than 24 hours) of xylene and does not cause excessive tissue brittle, contrary make some comparative quality and tough structure organization: such as uterine fibroids and other pretty good cut) pathological tissue sections, but when the temperature exceeds 40 ℃ after xylene, organizations can easily cause brittle hair. 5, through the wax (56-60 ° incubator) (Step 4-5) 80% alcohol (≤1h) →→ 95% alcohol (≈1h) →→ 100% alcohol (1) (2) (45-100min ) →→ 1/2 + 1/2 xylene alcohol (30min) →→ xylene (35min + 30min) →→ 1/2 + 1/2 xylene wax (45min) →→ wax (1) (2) ( 1-2h) 6, embedded in a variety of stationary phase, by a chemical substance, known simply fixative; composed by a variety of chemical substances are known as mixed fixative. Formaldehyde is the most common kind of pure fixative, its main role is to organize the organization hardening and corrosion, strong penetration of the tissue, fixed uniform, less tissue contraction, to maintain the natural morphology of cells stained clear colors. But after falling into place formaldehyde can disassemble, produce formic acid, the solution is acidic, the impact of nuclear staining. Therefore, the addition of certain small doses of formaldehyde solution in alternate suppliers neutralizer tissue sections, the solution was neutral (or weakly alkaline), referred to as neutral formaldehyde. Neutral formalin fixative are mixed. Neutral formalin-fixed tissue sections tissue supply, more conducive to maintaining tissue antigens, help detect immunohistochemical markers, and to avoid the formation of formaldehyde pigments, but can not save the organization uric acid salt crystals. Ethanol is also a simple fixing agent, fixing and dehydration effect, but its penetration is weak, fixed slow, easy to make tissue fragile, Further, ethanol precipitation enable nuclear protein, a nuclear protein is precipitated can be dissolved water is not conducive chromosomes fixed tissue sections, is a poor nuclear coloring affect morphological observation, it is generally not as conventional ethanol fixative applications. Frozen tissue sections _ voyage teaching (certified) _ Binzhou tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_48817160.html), for more information about the company.