Making a biopsy. Drawn (1) Material Fresh: derived tissue pathology report more fresh as possible, usually in human tissue from the body, the animal tissues fixed quickly after death, in order to ensure that the original morphological structure. (2) the size of the organization blocks: the ideal volume of tissue blocks 2.0cm × 2.0cm × 0.3cm, so fixative to penetrate quickly and evenly within the organization, but according to the producer of the material and for different purposes, tissue blocks The ideal size is also different, such as the production of pathological external examination, research section, the organization blocks can take 0.1 ~ 0.2cm thin you can, so you can shorten the time fixed dehydration transparent, if the production of teaching thick slices take 0.3 ~ 0.5cm, so You can create the same wax block teaching more slices. (3) Do not squeeze tissue block: tissue blocks were cut with sharp scissors biopsy should not move back and forth filing cutting. Do not overtighten gripping tissue, so as to avoid leaving squeeze tissue, cell deformation. 7 staining (3) 50 ~ 60 ℃ cooled glycerol was added. Glacial acetic acid (4) overnight, dark place. With a good liquid should be purple, black. Harris hematoxylin in with a good, add an equal amount of Ehrlich hematoxylin (hematoxylin with Ehrlich Law: A: hematoxylin 2g + ethanol 100ml + glycerin 100ml; B: potassium alum 20g + distilled water 100ml: A + B + acetic acid 5ml ). In preparation hematoxylin added Ehrlich hematoxylin obvious, Ehrlich hematoxylin solution is naturally oxidized mature nature more stable, it can become an effective stabilizer Harris hematoxylin, the solution is not easy to produce precipitation and oxide film, to avoid precipitation of impurities on sliced especially nuclear contamination biopsy What does it mean to achieve a stable solubilization, uniformly colored effect; constantly replenish its slow chromophore and auxochrome biopsy, can effectively control due to aging Harris hematoxylin staining force reduction problem, greatly extending the life of hematoxylin, the average life of 2 times the Harris hematoxylin. Both hematoxylin played a complementary role, both to ensure speed and staining of tissue selectivity, but also to overcome the traditional Harris hematoxylin easily form a precipitate, the disadvantage is easy to aging. Through the above key improvements, HE staining quality Ming hepatitis PT, APTT, TT prolonged, FIB reduce obvious. Pathology practices | voyage teaching (certified) | Anyang biopsy provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_44549635.html), for more information about the company.