7 staining (3) 50 ~ 60 ℃ cooled glycerol was added. Glacial acetic acid (4) overnight, dark place. With a good liquid should be purple, black. Harris hematoxylin in with a good, add an equal amount of Ehrlich hematoxylin (hematoxylin with Ehrlich Law: A: hematoxylin 2g + ethanol 100ml + glycerin 100ml; B: potassium alum 20g + distilled water 100ml: A + B + acetic acid 5ml ). In preparation hematoxylin added Ehrlich hematoxylin obvious, Ehrlich hematoxylin solution is naturally oxidized mature nature more stable, it can become an effective stabilizer Harris hematoxylin, the solution is not easy to produce precipitation and oxide film, to avoid precipitation of impurities on sliced especially nuclear contamination pathological figure, reaching a stable solubilization, uniformly colored effect; constantly replenish its slow chromophore and auxochrome pathology practices, can effectively control due to aging Harris hematoxylin staining force reduction problem, greatly extending the life of hematoxylin, the average life of 2 times the Harris hematoxylin. Both hematoxylin played a complementary role, both to ensure speed and staining of tissue selectivity, but also to overcome the traditional Harris hematoxylin easily form a precipitate, the disadvantage is easy to aging. Through the above key improvements, HE staining quality Ming hepatitis PT, APTT, TT prolonged, FIB reduce obvious. To cut and a knife slicing, first of all have a sharp knife. Brothers is engaged in one of the most fundamental and technical personnel slicing technique, knife grinding good or bad, is directly related to the quality of the slice. Sharpening the way everyone is different, but they all require biopsy force uniform, so that the knife-edge and full access to the grindstone, both hands firmly on the blade should point, rather than on the back of a knife. Knife grinding application once again, only 10 to 15 minutes each, over a long period of sharpening, easy to grind the blade has worn away, check whether the sharp knife and try to touch the knife-edge method of hand is not very Science pathological production process, not only can not prove that the knife is really sharp, and easy to damage the blade, the easiest way is to try to get a wax block cut after a good look at each mill. After each wear a good knife, cover with a lid whetstone should prevent dust off the grindstone, with gray grindstone sharpening, knife will produce many small gaps. Many units now have bought Grinder, Grinder for opening blade and grinding gap really good, but because of the angle and time is not easy to accurately grasp the knife, so the effect is not ideal. According to our experience, the real hard to grind out by machine blade. Disposable blade largely solved this problem. As with disposable blades, blade must be replaced. A knife cut into small samples should not exceed 20 to 25 wax block, cut into large specimens should not exceed 10 to 15 wax blocks. Pathological case, Henan pathology, teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future! You can also access (www.yuanhang369.com/supply_details_37717005.html), for more information about the company.