① fresh cryostat sections of frozen, thickness 6 ~ 8μm, flat on the coverslip. ② drop staining: Frozen sections will have coverslips (sample side up) flat on the preheated dish (bottom cushion a wet filter paper), the incubation medium was added dropwise to cover all tissues, the temperature at 37 ℃ and incubated for 45 to 60 minutes (liver, heart tissue in 15 minutes). ③ rinse twice in saline (Sway tissue sections, to prevent off-chip). ④10% saline formalin fixed 10 minutes. ⑤15% alcohol immersion 5 minutes (in other secondary). ⑥ glycerogelatin mounted. ⑸ results: strong activity at blue particles (double A precipitation), the formation of purple monomethyl lower activity. In the light microscope, the enzyme activity in the lobular distribution is obviously zoning, surrounding band is stronger than the central belt. In cardiomyocytes longitudinal section, enzyme granules aligned along the long axis of myocardial cells, cardiomyocytes in the space adjacent to intercalated disk. Display glycogen periodic acid Schiff (PAS) reaction: ⑴ principle: Glycogen is a polysaccharide animal animal tissue sections, colorless, abundant in the liver and muscle cells cytoplasm. PAS reaction (Periodic acid schiff Reaction) can generate a purple material flow at the site of tissue sections where glycogen, which will it be displayed. RAS reaction in two steps: First, a strong oxidant periodic acid (Periodic acid, formula HIO4), the CC bond glycogen glucose molecules open, CHOH-CHOH oxidative tissue slice production, generating two aldehyde, then Schiff reagent leuco magenta sulfite molecule binding to sugar aldehyde, to generate a new purple complex. HIO4schiff polysaccharide aldehyde reagent purple reaction product (oxide) (reagent preparation and histological methods are detailed technical monograph) ⑵ Results: purple glycogen granules. Tissue sections make the voyage _ teaching (certified) _ Xuchang tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_38125455.html), for more information about the company.