1, fixed with a tissue protein coagulation or precipitation effect, so can inhibit the glycolytic enzymes activities and reproductive tissue sections supply of bacteria, thus rendering the fixed effects (1) 10% formalin solution for organizations: 1 part formaldehyde + 9 parts of distilled water on a liquid market for sale formaldehyde solution containing about 37-40% formaldehyde tissue sections suppliers, 10% formalin solution actually contains only 3.7-4.0% formaldehyde Scope: a variety of organizations. However, in 10% formalin fixed tissue, nuclear staining better, and cytoplasmic staining of poor treatment: Organization blocks generally fixed for more than 24h, for a long time can even be used to block the long-term preservation organization. Short-term fixed tissue blocks without washing, can be directly transferred to 70% alcohol dehydration (2) Bouin's solution: saturated picric acid solution (1.22%) 75ml 25ml formaldehyde, acetic acid solution 5ml Scope: a variety of animal tissues and plant tissues apical, animal tissue fixative good deal: fixed 24h, can also be saved in this long-term solution. Without running water or washed directly into the 70% ethanol dehydration. 4, transparent (56-60 degrees thermostat) in order to make paraffin dipped into the tissue blocks, you must go through a mixing both with a dehydrating agent, but also media compatibility with paraffin material, this process is called transparent. Currently the best transparent agent is xylene tissue sections, at room temperature, if not too long (more than 24 hours) of xylene and does not cause excessive tissue brittle, contrary make some structure comparison of quality and tough Organization: for example uterine fibroids cut quite good), but when the temperature exceeds 40 ℃ after xylene, can easily cause tissue brittle. 5, through the wax (56-60 ° incubator) (Step 4-5) 80% alcohol (≤1h) →→ 95% alcohol (≈1h) →→ 100% alcohol (1) (2) (45-100min ) →→ 1/2 + 1/2 xylene alcohol (30min) →→ xylene (35min + 30min) →→ 1/2 + 1/2 xylene wax (45min) →→ wax (1) (2) ( 1-2h) 6, paraffin-embedded tissue sections | voyage teaching (certified) | Xuchang tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_37716965.html), for more information about the company.