4 embedding small piece of tissue should be linearly embedded, massive tissue when embedding should be flattened to prevent slicing incomplete and chaotic situation caused by misdiagnosis chip organization. For some wall, the wall protruding tissue or tissue should be buried legislation that embedded along the longitudinal projection bread buried in order to observe its various levels. In order to ensure the smooth sections should be used having a melting point of 60 ~ 62 ℃ paraffin embedding performed. 5 slice sliced ??slices as possible while maintaining complete, thin and uniform Fujian biopsy, usually 2 ~ 3μm Hangzhou biopsy, for lymph nodes, tonsils and other foods rich in lymphocytes in tissue sections require less than 2μm, can not appear too thick and sliced cracks, etc., required endoscopic biopsy during specimen cut out section 4 above. Avoid the emergence of parallel slices ripples, folds broken empty, uneven thickness, wax curling phenomenon. Grilled slices and dewaxing baked pieces, usually in temperature inside the baking sheet 60 ℃ about 0.5 to 1 hour, the temperature is too high, the slice will cause cell contraction; time is too short (less than 20 minutes), likely to cause off-chip. Dewaxing is also important, if not clean dewaxed tissue biopsy, sliced ??difficult uneven coloring or shading biopsy, therefore, dewaxing with xylene should be changed frequently, usually with three xylene per 500ml of liquid handling 500 After replacing the appropriate time slices. When the temperature is below 18 ℃, must be sliced ??out from the oven immediately placed dewaxing in xylene or xylene into the temperature inside to warm up (about 37 ℃) dewaxing not exceed 60 ℃ . Then purified by high concentrations of alcohol to the low concentration of alcohol wash xylene, to water. Jiangxi pathology, pathology, teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future! You can also access (www.yuanhang369.com/supply_details_42723035.html), for more information about the company.