5 slices respond carefully slicing machine to do a comprehensive inspection and clamping screws can not be loose, hand-hand wheel speed should be uniform pathology, not too fast and too fast, so jump knife and body instability, slicer erase wax should be patient debris and oil, and lubricating drops maintenance stand. Be sure to keep a sharp knife, the blade reached no gap, roll edge, under the eyepiece was fine sawtooth pattern like Hangzhou biopsy, the angle between the knife and the appropriate wax block at 10 degrees. Drift films can not have bubbles or folds, to fully flattened. Mounted flat sheet requirements, may be floating in 95% ethanol, and then transferred to warm water to increase its surface tension, better maintain slices flat trace. Maintain the water temperature 49 ℃ ~ 51 ℃, low temperature Zhejiang biopsy, fine wrinkles can not be softened, the water temperature is too high will cause tissue cells spread. Keep the water clean, avoid residual water Fujian biopsy tissue debris to prevent contamination during the backing sheet. Fixed washing after washing (10 to 20 minutes), is often overlooked by many people, but water is not complete, likely to cause off-chip and stain is not bright. To be done on tissue immunohistochemistry, but should not be overlooked. Formalin is not conducive to the preservation of the tissue block antigen. Dehydration and dehydration is the use of so-called transparent dehydrating agent will displace the water within the organization to facilitate the infiltration of organic solvents, a process called dehydration. Dehydration is complete, is directly related to whether the organization can be fully transparent, and excessive dehydration (due to the organization of pure alcohol in a long time, the occurrence of tissue texture change) is likely to cause tissue brittle. The cause of the organization as well as crisp warm (temperature exceeds 35 ℃), within the dehydrating agent added acetone, xylene excessive use wax dipped in paraffin and so on. Hangzhou pathology | voyage teaching (certified) | pathology provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) (www.yuanhang369.com) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, the maximum meet customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage. You can also access (www.yuanhang369.com/supply_details_44549625.html), for more information about the company.