3, show alkaline phosphatase (APL), cobalt calcium method (Alkaline phosphohydrolase) ⑴ positioning enzyme and its biological significance: ALP in an alkaline environment can catalyze hydrolysis of phosphate esters of various alcohols and phenols, phosphate cross participation membrane transport process tissue sections, and phosphate transfer effect, it is more active in the cell membrane transport, such as capillary endothelial cell tissue sections Xinxiang supply, renal proximal tubule brush border tissue sections offer Xinxiang, intestinal sticks border, etc. Xinxiang pathological tissue sections, the higher activity of the enzyme. ⑵ Principle: ALP phosphate substrate (e.g. β- glycerophosphate, etc.) decomposition of phosphate ions, the latter capture the incubation medium to generate calcium chloride, calcium phosphate precipitation, but the precipitate non-metal salt, to be added cobalt nitrate, cobalt phosphate precipitate it generates, due to colorless, and then the processing required by ammonium sulfide, cobalt sulfide formed brown precipitate is displayed. Showed maximum activity PH9.2-9.4 environment. Reaction is as follows: ALPCa ++ β- glycerophosphate phosphate ions Ca2 (PO4) 2 Co (NO3) 2 (NH4) 2S CO2 (PO4) 2CoS ↓ (colorless) (brown) ⑶ results: the enzyme active site for the brown-black CoS precipitation. Note ① community block fixation, dehydration, dipping wax transparency and quality directly affects the slice preparation. The difficulties encountered during the first slice should be noted from the above link to find the reason before the slice. ② quality slicer is an important prerequisite for the preparation of high-quality slices. To use good quality slicer, slice standardized, well-maintained slicer. ③ via endoscopy, biopsy and other small communities acquired should intermittent multi-faceted serial sections (typically at least six wax preparation, if necessary, prepare for more photos). Need for special staining, immunohistochemistry and other cases, you can pre-wax spare. ④ slice operations personnel should carefully guard against being cut dicer. Embedding (1) manual routine embedding: Meet the above "(i) manual" item. (2) when embedding machine with a community, according to the provisions relating to the operation of the manufacturer's instructions. Xinxiang tissue sections supply _ _ voyage teaching tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!