⑵ dehydration: is the tissue cells contained moisture removed. Usually with ethanol (Alcohol, alcohol), the concentration of the delivery rise to 100%. This process also enables organizations to block further hardened. ⑶ Transparency: with both pure alcohol soluble organic solvent can melt wax, alcohol will replace the organization so immersed in paraffin. Usually with xylene (xylene) is transparent agent. ⑷ Baptist wax: The temperature inside the conduct. The organization has been transparent pieces into heated to melt the wax and let soak paraffin tissue, as a support medium. ⑸ embedding: The tissue blocks have dipped into the hot melt wax paraffin embedding, the rapid condensation Xinxiang animal tissue sections, it is a wax-embedded tissue decision. ⑹ sections: The slicer. Commonly used rotary slicer have flat push type. The easy-to-use rotary cut serial sections. Slice thickness adjustment purposes with the producer. Mostly teaching specimen thickness 5 ~ 6μm. ⑺ patch to dry: the warm paraffin flattened, moving on a glass slide, dried, can be processed for staining. After staining with gum, sloping piece sealing lid, can be permanently stored. A display of nucleic acid green faction Well Ning (Methyl green-pyronln) staining: ⑴ Principle: A green and basic dyes are sent Ning Well tissue sections, the two nucleic acids (DNA and RNA) can be dyed separately, the mechanism may be that the two nucleic acid molecules are polymers Xinxiang pathological tissue sections, but with varying degrees of polymerization. A green has two uncharged amino, and only one faction Well Ning. Therefore, in the mixed dye, the result of competition between the two is that the higher the degree of polymerization A green easy and DNA-binding blue-green, but rather is associated with lower faction Well degree of polymerization of RNA binding is red. (Reagent preparation and histological methods are detailed technical monograph) ⑶ results: nuclear DNA was in the blue-green, nucleolar and cytoplasmic RNA was pink. Xinxiang tissue sections supply voyage teaching (certified), tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.