2, showing the enzyme glucose-6-phosphate (G-6-Pase) activity Lead Law: (Glucosel-phosphate phosphohydrolase) locate its biological significance ① enzymes: the enzyme primarily located in the endoplasmic reticulum, the endoplasmic reticulum marker enzyme. G-6-Pase involved in glucose metabolism, glucose-6-phosphate can be hydrolyzed to release glucose and phosphate. ⑵ Principle: G-6-Pase the substrate (glucose-6-phosphate potassium salt) hydrolysis of phosphate ions, the latter being in the incubation mixture of lead nitrate captured, the final reaction was pelletized lead sulfide precipitation, the reaction Formula as follows: enzyme Pb (NO3) 2 + H2O glucose-6-phosphate, potassium phosphate Glucose + ⑶ Results: the enzyme active site as a brown lead sulfide particles precipitate. In the light microscope, G-6-Pase active particles more evenly distributed in the cytoplasm. Slice preparation routine operating procedures and requirements (i) The sequence block associations were: ① washing, ② dehydration, ③ transparent Henan pathological tissue sections, ④ dip wax, ⑤ ⑥ embedding and sectioning. (Ii) Preparation and HE communities sliced ??dyeing process using ethanol, acetone, xylene, paraffin and other flammable, toxic, have someone manage tissue sections, 2m or less do not have to suffer a fire flame Henan tissue sections offer local environment should have good ventilation and fire-fighting facilities. (Iii) regular slice manual (step sequence and duration of each step) 1. Wash: Rinse with water associations have fixed block 30min. 2. Dried (room temperature) (1) ethanol - formaldehyde (AF) were fixed 60 ~ 120min (2) 80% ethanol, 60 ~ 120min (3) 95% ethanol Ⅰ 60 ~ 120min (4) 95% ethanol Ⅱ 60 ~ 120min (5 ) ethanol Ⅰ 60 ~ 120min (6) ethanol Ⅱ 60 ~ 120min (7) ethanol Ⅲ 60min [Note] ① inadequately fixed into the community does not suffer from dehydration process. ② for dehydration reagent volume should be at least 5 to 10 times the total volume of the block association. ③ Since the low concentration of ethanol to progressively move into high concentrations of ethanol dehydration. ④ dehydrating reagents should promptly filter replacement (500ml ethanol can be used in 500 communities block dehydration; ethanol turns blue when adding copper sulfate, suggesting that needs to be replaced). ⑤ larger community dehydration block longer than the smaller supply Henan tissue sections, should be separated both dehydration. ⑥ societies for the time not too long inside ethanol (to avoid hardening). ⑦ acetone dehydration strong performance, block associations will shrink too, hard and brittle, not to replace ethanol. Henan tissue sections Quote | tissue sections | voyage teaching provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!