2, showing the enzyme glucose-6-phosphate (G-6-Pase) activity Lead Law: (Glucosel-phosphate phosphohydrolase) positioning and its biological significance ① enzymes: the enzyme is mainly located in the endoplasmic reticulum, the endoplasmic reticulum marker enzyme. G-6-Pase involved in glucose metabolism in tissue sections offer Henan, the hydrolysis of glucose-6-phosphate and releases glucose and phosphate. ⑵ Principle: G-6-Pase the substrate (glucose-6-phosphate potassium salt) hydrolysis of phosphate ions Henan animal tissue sections, the latter being the incubation medium of the captured lead nitrate, the final reaction was pelletized PbS precipitation, the reaction is as follows: enzyme Pb (NO3) 2 + H2O glucose-6-phosphate, potassium phosphate, glucose + ⑶ results: the enzyme active site brown lead sulfide particles precipitate. In the light microscope, G-6-Pase active particles more evenly distributed in the cytoplasm. ① general embedded with hard wax embedded tissue sections Henan pathology, namely the melting point of paraffin wax 60-62 ℃. Consistent with paraffin melting paraffin embedded melting and dipping wax to use. Paraffin wax dip slightly higher temperature medicine temperature ② embedded use, maintained at 65-68 ℃ tissue sections, but not too high, otherwise easily lead to burn tissue, and experimental results. ③ embedding procedure to quickly avoid the tissue block has not buried properly before melting wax solidifies. ④ mucosa, wall, skin tissue must be perpendicular to the ground embedding cassettes, buried or standing upright habit called packets. Vessel wall should be buried upright. ① knife slicing want sharp knife without defect, to cut out the entire sheet. At present, many with a disposable blade slices, slice of good quality. If using heavy-duty all-steel knife, they have often ground to maintain its sharp, no defect. ② bone and other tissue calcification calcium must be removed, and then after each step of the processing described before slicing. Henan tissue sections produced voyage teaching (certified), tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.