The following briefly describes several aspects from the principle of common staining technique: a display of succinate dehydrogenase (SDH) activity nitro -BT law: (Succinate dehydrogenase) ⑴ positioning enzymes and biological significance: succinic off mitochondrial marker enzyme catalase, which is present in all cells of aerobic respiration, and mitochondrial closely. Without coenzyme and enzyme itself has flavoprotein (FP, Flavoprotein) supplemented groups. In the group of reaction is often used to reflect the situation in the citric acid cycle. ⑵ Principle: The final step of the above reaction principle, nitro -BT (Nitro-blue tetrozolyum, tetrazolium) systems can vary tetrazolium ring compounds, when it accepts the hydrogen itself is reduced to a colored precipitate the product ( A) (Formazan). 3. Transparency: not because of dehydration and embedding media compatibility, thus embedding application before embedding medium compatible with chemical agents will organize displace the dehydrating agent, the process shall be transparent. In this process, the organization quickly ethanol is the solvent medium (i.e. transparent agent, such as xylene) substituted, its refractive index close to the refractive index of tissue protein, the light can be transmitted, the tissue blocks showed varying degrees transparent. Xylene is a colorless, transparent liquid, transparent and strong ability, but easy to make tissue blocks shrink, harden brittle tissue sections Henan factory, so the tissue blocks in xylene time not too long, in order to achieve transparency for the degree of organization (organization block presents brown or dark yellow to transparent). Another similar Henan tissue sections suppliers, benzene, toluene and xylene nature, but also for transparent agent. Both blocks of tissue shrinkage less easy to make tissue hardening brittle, but transparent slow, takes about 60min. Chloroform is not as transparent ability xylene and volatile Henan tissue sections offer fast and easy to absorb moisture, and more for large tissue mass (thick 1cm) transparent, clear time for 12-24h. Henan tissue sections produced | voyage teaching (certified) | tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.