① fresh cryostat sections of frozen, thickness 6 ~ 8μm, flat on the coverslip. ② drop staining: Frozen sections will have coverslips (sample side up) flat on the preheated dish (bottom cushion a wet filter paper), the incubation medium was added dropwise to cover all tissues, the temperature at 37 ℃ and incubated for 45 to 60 minutes (liver, heart tissue in 15 minutes). ③ in saline rinse twice (Sway prevent off-chip). ④10% saline formalin fixed 10 minutes. ⑤15% alcohol immersion 5 minutes (in other secondary). ⑥ glycerogelatin mounted. ⑸ results: strong activity at blue particles (double A precipitation), the formation of purple monomethyl lower activity. In the light microscope, the enzyme activity in the lobular distribution is obviously zoning, surrounding band is stronger than the central belt. In cardiomyocytes longitudinal section, enzyme granules aligned along the long axis of myocardial cells, cardiomyocytes in the space adjacent to intercalated disk. 3, show alkaline phosphatase (APL), cobalt calcium method (Alkaline phosphohydrolase) ⑴ positioning enzyme and its biological significance: ALP in an alkaline environment can catalyze hydrolysis of phosphate esters of various alcohols and phenols, phosphate cross participation membrane transport process Xinxiang tissue sections suppliers, and the role of phosphate transfer Xinxiang tissue sections offer, so the more active transport in the cell membrane, such as capillary endothelial cells, renal proximal tubule brush border of intestinal border on sticks, etc., high activity of the enzyme. ⑵ Principle: ALP phosphate substrate (e.g. β- glycerophosphate, etc.) decomposition of phosphate ions, the latter is incubated in calcium chloride solution to generate the calcium phosphate precipitation captured tissue section, but the non-metal salt precipitate, to be added cobalt nitrate xinxiang tissue slice processing to generate cobalt phosphate precipitation, because colorless, then processing required by ammonium sulfide, cobalt sulfide formed brown precipitate is displayed. Showed maximum activity PH9.2-9.4 environment. Reaction is as follows: ALPCa ++ β- glycerophosphate phosphate ions Ca2 (PO4) 2 Co (NO3) 2 (NH4) 2S CO2 (PO4) 2CoS ↓ (colorless) (brown) ⑶ results: the enzyme active site for the brown-black CoS precipitation. Xinxiang tissue sections manufacturers, tissue sections, teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!