① fresh cryostat sections of frozen, 6 ~ 8μm thick tissue sections, flat on the coverslip. ② drop staining: Frozen sections will have coverslips (sample side up) flat on the preheated dish (bottom cushion a wet filter paper), the incubation medium was added dropwise to cover all tissues, the temperature at 37 ℃ and incubated for 45 to 60 minutes (liver, heart tissue in 15 minutes). ③ in saline rinse twice (Sway prevent off-chip). ④10% saline formalin fixed 10 minutes. ⑤15% alcohol immersion 5 minutes (in other secondary). ⑥ glycerogelatin mounted. ⑸ results: strong activity at blue particles (double A precipitation) Xinxiang tissue slices manufacturers, forming a purple monomethyl lower activity. In the light microscope, the enzyme activity in the lobular distribution showed a zoning Xinxiang tissue sections suppliers around the band is stronger than the central zone. In cardiomyocytes longitudinal section, enzyme granules aligned along the long axis of myocardial cells, cardiomyocytes in the space adjacent to intercalated disk. ⑵ hollow organs: esophagus, stomach, intestines, etc. should first cut, the mucosa facing tile in cork board and nail with a pin on the edge of the wood specimens, and then placed in fixative, mucosal surface covered with cotton or gauze, or mucosal face down dip and fixative. ⑶ Brain: The whole brain tissue fixation, downtown bottom-up, the first in a long suture through the basilar artery rings, then holding hands has been injected into the brain tissue fixative container, then the suture ends up slightly lifting, by means of the buoyancy of water suspended in the brain tissue fixative, then the suture is fixed to the wall of the container. Brain tissue fixation required a longer time, usually 10-15 days, fixed one week should be replaced once a fixative. ⑷ eye: eye surface structure of dense tissue sections offer Xinxiang, fixative not penetrate, should be fixed by doing a sagittal diameter corneal incision of the cornea, the small pieces of paper rolled into a given thickness inserted into the incision, so fixative by This incision into the eye, in order to facilitate fixation. ⑸ non-small overall organ tissue fixative directly with a sufficient amount can be fixed. Xinxiang tissue sections offer voyage teaching (certified), tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.