7, after the separation with tap water or a few drops of ammonia alkaline until the nuclei blue. This step can also be called "Blue-oriented." 8, into distilled water to wash away the alkaline water. 9, the 70% ethanol → 80% ethanol for 5 minutes. 10, into the dye eosin staining 30 seconds to a few minutes. 11 to 95 percent alcohol production in Iraq red dichroic tissue sections, to the cytoplasm, connective tissue was pink. 12, the upstream dehydration: sections were stained after opaque due to tissue containing water, a fine structure which can not be observed clearly. Therefore, the water shall alcohol levels within the organization through the gradual replacement → ethanol, and finally into the water without a transparent agent. 13. Transparency: the xylene agents, secondary (each a few minutes). 14, remove the slices: the surrounding tissue to wipe the excess xylene, add coverslip after sealing gum drops. Results: The nuclei blue-violet, cytoplasm, collagen fibers, muscle fibers pink, bright red eosinophilic granular elastic fibers were bright pink. Transparent (1) Xylene Ⅰ 20min (2) xylene Ⅱ 20min (3) Xylene Ⅲ 20min [Note] ① volume xylene should be 5 to 10 times more than the total volume of the block association. ② Societies transparent block in xylene time not too long (to prevent community hard, brittle), and depending on its size and different categories of society; associations appear transparent to brown or dark red. ③ xylene should be filtered and replaced. Tissue sections manufacturers transparent when not significant after ④ community through xylene extent appropriate treatment, often suggesting that the association is not fully fixed or dehydration, you should find out the reasons and appropriateness of treatment. Baptist wax (a) paraffin Ⅰ (45 ~ 50 ℃) 60min (2) paraffin Ⅱ (56 ~ 58 ℃) 60min (3) paraffin Ⅲ (56 ~ 58 ℃) 60min [Note] ① melted paraffin must have the person responsible, pathological tissue sections must be in the box or melting wax bath (70 ℃), does not suffer from a flaming fire by heating. ② melting wax volume should be at least 5 to 10 times the total volume of the block associations. ③ communities block by xylene degree before dipping into the wax after due process transparent tissue sections, should be as clear cut after xylene community block surface into molten wax. ④ appropriate time Baptist wax, wax dipped too short inadequate (community too soft), when long association brittle. ⑤ melting wax should be filtered and replaced. Tissue sections _ voyage teaching process (certified) _ tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.