7, after the separation with tap water or a few drops of ammonia alkaline until the nuclei blue. This step can also be called "Blue-oriented." 8, into distilled water to wash away the alkaline water. 9, the 70% ethanol → 80% ethanol for 5 minutes. 10, into the dye eosin staining 30 seconds to a few minutes. 11 to 95 percent alcohol Iraq red color separation, to the cytoplasm, connective tissue was pink. 12, the upstream dehydration: sections were stained after opaque due to tissue containing water, a fine structure which can not be observed clearly. Therefore tissue sections suppliers, water shall alcohol levels within the organization through the gradual replacement → ethanol, and finally into the water without a transparent agent. 13. Transparency: the xylene agents, secondary (each a few minutes). 14, remove the slices: the surrounding tissue to wipe the excess xylene, add coverslip after sealing gum drops. Results: The nuclei blue-violet, cytoplasm, collagen fibers, muscle fibers pink, bright red eosinophilic granular tissue sections, elastic fibers were bright pink. When sliced ??sliced ??③ wax block should trim neatly around, especially on the lower edge, requiring parallel, otherwise difficult to even sliced ??into a strip. ④ to be fixed to the holder block at room temperature and cut tissue should be kept cool in an ice box Henan tissue sections, especially in the summer, in order to maintain the hardness of the wax block. ⑤ To reduce knife block with heat generated in tissue slices during paraffin maintain proper hardness, while cooling with ice chips can often tissue blocks and knife. ⑥ as excellent low temperature or paraffin, cut pieces not available in the appropriate section heated paraffin blocks (such as with a finger or with hot wax block pressed gauze, with the temperature or heat heating hot gauze). ⑦ slides should be kept clean and smear protein glycerin (glycerol + 1 part 1 part egg mix to) Zhengzhou tissue sections, to prevent the off-chip (immunohistochemical staining, the slides should be coated with gelatin, resin adhesive, APES, polylysine and other anti-off tablets, in order to increase the fastness of adhesion of the slice, to prevent the off-chip.). ⑧ wax block after cut, sealed with molten paraffin section, in order to facilitate long-term preservation. Tissue sections processed tissue sections, teaching voyage by voyage Teaching Equipment Co. Xinxiang. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!