3. Dehydration transparent specimen after fixation and washing, tissue contains more moisture, the organization of water must be replaced within a block out, this process is called dehydration. Whether it is pathological paraffin what it meant, or with collodion slice must remove moisture contained in biopsy tissue, due to the aqueous tissue and paraffin embedding collodion and other incompatible materials commonly used dehydrating agent for a series of different concentrations of ethanol. Step dehydration are: 80%, 90%, 95%, 100% ethanol at various concentrations 2 hours biopsy practices, this process can be controlled to complete dehydration. Acetone is also a strong dehydrating agent dewatering capacity, but because of dehydration are strong, severe contraction of the organization, in the production of research, teaching sections generally do not have the reagents. Due to ethanol, acetone and other insoluble in paraffin wax, paraffin wax can be dissolved to go through a process known as alternative solvents and transparent. Commonly used transparent agent xylene, chloroform, methyl salicylate and the like. 4 embedding small piece of tissue should be linearly embedded, massive tissue when embedding should be flattened to prevent slicing incomplete and chaotic situation caused by misdiagnosis chip organization. For some wall, the wall protruding tissue or tissue should be buried legislation that embedded along the longitudinal projection bread buried in order to observe its various levels. In order to ensure the smooth sections should be used having a melting point of 60 ~ 62 ℃ paraffin embedding performed. 5 slices as possible while keeping sliced ??slices intact, thin and uniform, usually 2 ~ 3μm pathology report, for lymph nodes, tonsils and other foods rich in lymphocytes in tissue sections require less than 2μm, can not appear too thick slices and fissures, etc. endoscopic biopsy during specimens cut out four facets need more. Avoid the emergence of parallel slices ripples, folds broken empty, uneven thickness, wax curling phenomenon. Pathology reports, pathology, teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!