Making a biopsy. Drawn (1) Material Fresh: derived tissue biopsy more fresh as possible, usually in human tissue from the body, the animal tissues fixed quickly after death, in order to ensure that the original morphological structure. (2) the size of the organization blocks: the ideal volume of tissue blocks 2.0cm × 2.0cm × 0.3cm, so fixative quickly and evenly penetrate the internal organization of pathology report, but according to the producers of materials and different purposes , tissue blocks are different than the ideal volume, such as the production of pathological external examination, research slice it thin tissue blocks can then take 0.1 ~ 0.2cm, which can shorten the time fixed dehydration transparent, if the production of teaching thick slices of 0.3 to 0.5 cm, so you can create the same paraffin block teaching more slices. (3) Do not squeeze tissue block: tissue blocks were cut with scissors to be sharp, cutting can not move back and forth filing. Do not overtighten gripping tissue, so as to avoid leaving squeeze tissue, cell deformation. 4. Baptist wax-embedded (1) immersed in paraffin tissue, instead of the transparent tissue containing agents. (2) embedding: the organization after dipping into melted wax, paraffin wax, paraffin solidification, i.e. the package in which the organization, said the wax block, this process is called embedding. 5. Slice and patch (1) trimming wax block: visual size of their organization, the organization of about 0.1 ~ 0.2cm at the edge, cut more than wax part, otherwise easily lead to uneven shrinkage organization. (2) ready for slicing tools: knife, brush, eye tweezers (bent), drift baking temperature controller (3) installation of wax blocks: the repaired wax block mounted on a metal or wooden candle holders on. (4) installation knife: the knife mounted on the stage slicer knife, the knife fastening screws tightened on stage, so no vibration when sliced, to maintain a certain slice thickness. (5) the thickness of the slice: the slicer thickness regulator engraved 0 ~ 50μm or 0 ~ 25μm, the thickness thereof can be arbitrarily selected biopsy, paraffin section of thickness is generally 4 ~ 6μm. (6) sections (7) stretched: ophthalmic tweezers tweezers with wax on the surface with a lightly tile 40 ~ 45 ℃ of pathology is what is meant by the tension of the water and the water temperature, the wax with a slightly wrinkled natural flattening. (8) patch, baking sheet: After slicing the water on the heated sufficiently flattened middle groan at the wax to pour over the water slides on the slide, placed in an oven at 60 ~ 65 ℃ or within slice drift bake oven temperature controller chip bake 15 to 30 minutes, removing melted paraffin tissue space. Pathological production _ _ pathology teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.