4. Baptist wax-embedded (1) immersed in paraffin tissue, instead of the transparent tissue containing agents. (2) embedding: the organization after dipping into melted wax, paraffin wax, paraffin solidification, i.e. the package in which the organization, said the wax block Kaifeng biopsy, this process is called embedding. 5. Slice and patch (1) trimming wax block: visual size of their organization, the organization of about 0.1 ~ 0.2cm at the edge, cut more than wax part, otherwise easily lead to uneven shrinkage organization. (2) ready for slicing tools: knife, brush, eye tweezers (bent), drift baking temperature controller (3) installation of wax blocks: the repaired wax block mounted on a metal or wooden candle holders on. (4) installation knife: the knife mounted on a knife slicer table, table knife fastening screws tightened, so that does not produce vibrations when biopsy slice, can maintain a certain slice thickness. (5) the thickness of the slice: the slicer thickness regulator engraved 0 ~ 50μm or 0 ~ 25μm, the thickness thereof can be arbitrarily selected, paraffin slice thickness is generally 4 ~ 6μm. (6) sections (7) stretched: ophthalmic tweezers tweezers with wax on the surface with a lightly tile 40 ~ 45 ℃, and by the tension of the water and the water temperature will be slightly wrinkled wax with natural flattening. (8) patch, baking sheet: After slicing the water on the heated sufficiently flattened middle groan at the wax to pour over the water slides on the slide, placed in an oven at 60 ~ 65 ℃ or within slice drift bake oven temperature controller chip bake 15 to 30 minutes, removing melted paraffin tissue space. To cut and a knife slicing, first of all have a sharp knife. Brothers is engaged in one of the most fundamental and technical personnel slicing technique, knife grinding good or bad, is directly related to the quality of the slice. Sharpening the way everyone is different, but they all require force uniform, so that the knife-edge and full access to the grindstone, both hands firmly on the blade should point, rather than on the back of a knife. Knife grinding application once again, only 10 to 15 minutes each, over a long period of sharpening, easy to grind the blade has worn away, check whether the sharp knife and try to touch the knife-edge method of hand is not very Henan Science sliced ??cases, not only can not prove that the knife is really sharp, and easy to damage the blade, the easiest way is to try to get a wax block cut after a good look at each mill. After each wear a good knife, cover with a lid whetstone should prevent dust off the grindstone, with gray grindstone sharpening, knife will produce many small gaps. Many units now have bought Grinder, Grinder for opening blade and grinding gap really good, but because of the angle and time is not easy to accurately grasp the Brothers Luoyang biopsy, so the effect is not ideal. According to our experience, the real hard to grind out by machine blade. Disposable blade largely solved this problem. As with disposable blades, blade must be replaced. A knife cut into small samples should not exceed 20 to 25 wax block, cut into large specimens should not exceed 10 to 15 wax blocks. Henan cases sliced ??| pathology | voyage teaching provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!