Frozen sectioning frozen section method is to use the tissue at low temperatures to freeze hard to achieve compliance with the requirements of sections. The need for frozen sectioning cryostat tissue sections produced, or in the general section with refrigeration facilities. Cryostat is a senior cryosections equipment. It has a cryostat, refrigeration units and slicer specimens are housed in the box. Similar effect cryostat refrigerator, the temperature can be adjusted within a certain range, its structure is conducive to maintaining a constant low temperature chamber, to reduce interference when the ambient temperature of the door opening. Rotary slicer handle mounted on the outside of the box, easy to manipulate slices. Advantages of frozen section method is: was handled, it can maximize their fresh state organization. And, because without dehydration, transparent, wax dip during the treatment of organic solvents, and the impact of wet boxes Baptist wax hot, even without immobilized. So well preserved organize various ingredients and enzyme activity of the cells, and therefore used this method in chemical research slice of tissue enzymes. 7, after separation drops of ammonia alkaline animal tissue sections with tap water or addend until the nuclei blue. This step can also be called "Blue-oriented." 8, into distilled water to wash away the alkaline water. 9, the 70% ethanol → 80% ethanol for 5 minutes. 10, into the dye eosin staining 30 seconds to a few minutes. 11 to 95 percent alcohol Iraq red color separation, to the cytoplasm, connective tissue was pink. 12, the upstream dehydration: sections were stained after opaque due to tissue containing water, a fine structure which can not be observed clearly. Therefore, the water shall at all levels within the organization by alcohol → ethanol gradual replacement tissue sections, and finally into the water without a transparent agent. 13. Transparency: tissue slices into xylene agent processes, secondary (each a few minutes). 14, remove the slices: the surrounding tissue to wipe the excess xylene, add coverslip after sealing gum drops. Results: The nuclei blue-violet, cytoplasm, collagen fibers, muscle fibers pink, bright red eosinophilic granular elastic fibers were bright pink. Tissue sections steps voyage teaching (certified), tissue sections provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.