Making a biopsy. Drawn (1) Material Fresh: derived tissue biopsy Luoyang more fresh as possible, usually in isolated human tissue biopsy after opening the body, animal tissues fixed quickly after death, in order to ensure that the original morphological structure. (2) the size of the organization blocks: the ideal volume of tissue blocks 2.0cm × 2.0cm × 0.3cm, so fixative to penetrate quickly and evenly within the organization, but according to the producer of the material and for different purposes, tissue blocks The ideal size is also different, such as the production of pathological external examination, research section, the organization blocks can take 0.1 ~ 0.2cm thin you can, so you can shorten the time fixed dehydration transparent, if the production of teaching thick slices take 0.3 ~ 0.5cm, so You can create the same wax block teaching more slices. (3) Do not squeeze tissue block: tissue blocks were cut with sharp scissors to be sliced ??Henan cases, cutting back and forth can not move file. Do not overtighten gripping tissue, so as to avoid leaving squeeze tissue, cell deformation. (5) the thickness of the slice: 0 ~ 50μm engraved or 0 ~ 25μm Histology slicer thickness regulator, the thickness thereof can be arbitrarily selected, paraffin slice thickness is generally 4 ~ 6μm. (6) sections (7) stretched: ophthalmic tweezers tweezers with wax on the surface with a lightly tile 40 ~ 45 ℃, and by the tension of the water and the water temperature will be slightly wrinkled wax with natural flattening. (8) patch, baking sheet: After slicing the water on the heated sufficiently flattened middle groan at the wax to pour over the water slides on the slide, placed in an oven at 60 ~ 65 ℃ or within slice drift bake oven temperature controller chip bake 15 to 30 minutes, removing melted paraffin tissue space. General pathology, pathology, teaching voyage by voyage Teaching Equipment Co., Ltd. Xinxiang City. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) strong, credible, in Xinxiang specimen model industry has accumulated a large number of loyal customers. The company better work attitude and constantly improving innovative ideas will lead the voyage into the brilliant teaching and you work for a better future!