2. Fixed (1) small tissue fixing method: This is the most commonly used method, small piece of tissue from the human or animal body removed, to be placed in a liquid fixative were immediately fixed, generally, the ratio of fixative to specimens with 1 : 4 to 20, but not too large tissue mass is too thick, otherwise it can not be quickly fixative penetration. Therefore, the size of the block of tissue typically 2.0cm × 2.0cm × 0.3cm. (2) injection, perfusion fixation: Some organizations block because the volume is too large or extremely difficult to penetrate inside the fixative, or need for a whole organ or whole animal is fixed. At this time should be fixed by injection or perfusion fixation. The fixative into a blood vessel, the vessel branches to reach the entire organization and the body, resulting in fully fixed. (3) steam fixation: a relatively small biopsy specimens and thick, can be osmium tetroxide or formaldehyde vapor fixation. Such as blood smears, blood smears should not dry before using osmium tetroxide or formaldehyde vapor contact fixed. The most commonly used fixative is 10% formalin fixative, and 95% ethanol fixative. Fixed washing after washing (10 to 20 minutes), is often overlooked by many people Xuchang pathology, and water is not complete, likely to cause off-chip and stain is not bright. To be done by immunohistochemistry of Anyang biopsy tissue, but should not be overlooked. Formalin is not conducive to the preservation of the tissue block antigen. Dehydration and dehydration is the use of so-called transparent dehydrating agent will displace the water within the organization to facilitate the infiltration of organic solvents, a process called dehydration. Dehydration is complete, is directly related to whether the organization can be fully transparent, and excessive dehydration (due to the organization of pure alcohol in a long time, the occurrence of tissue texture change) is likely to cause tissue brittle. The cause of the organization as well as crisp warm (temperature exceeds 35 ℃), within the dehydrating agent added acetone, xylene excessive use wax dipped in paraffin and so on. Puyang biopsy voyage teaching (certified), biopsy provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) is an industry leader in Henan Xinxiang, specimen model years, the company implementing the scientific management, innovation and development, the principle of honesty and trustworthiness, and satisfy customer needs. On the voyage instructional leadership with the staff warmly welcome all inquiries negotiations to create a better future teaching voyage.