Temporary and permanent piece of sheet material slices of sometimes for permanent preservation, or can not permanently stored, it is called a temporary piece. Reasons for making the use of temporary piece, probably because the material can not be permanently stored, or to facilitate the observation, only to deal with the material in a simple manner. Another possibility is the use of chemical agents easy changes can not be made a permanent piece. Another reason is the need to make a temporary piece Zuohuo body observed by the tissue or cells can remain alive for a short time to slide into. The activity tests such as pollen producers. Permanent piece of evidence is the remaining samples (slides), as proof study. Almost points based Cai Shuhua, a program for the charge material. Fixed material. Cut into thin slices. Staining. Mounted. 2. Rinse: The material removed since fixative frozen tissue sections, to be immediately flushed, which makes all the fixative all removed to prevent the dyeing some fixative, such as 10% formalin, Bouin's solution fixed material If the time is shorter, then need not be flushed. But the time is too long should also rinsed 3, dried (37 ° incubator) is the use of so-called dewatering dehydrating agent will displace the water within the tissue, to facilitate infiltration of the tissue slice processing of organic solvents, a process known as dewatering. Dehydration is complete, is directly related to whether the organization can fully transparent excessive dehydration (due to the organization of pure alcohol in a long time, the occurrence of tissue texture change) is likely to cause tissue brittle. The cause of the organization as well as crisp warm (temperature exceeds 36 ℃), within the dehydrating agent added acetone, xylene excessive use wax dipped in paraffin and so on. Generally, we always think crisp organization concerned with the high concentration of alcohol, while ignoring the relationship between low levels of alcohol, in fact, the low concentration of alcohol has strong penetration and easier than pure alcohol to penetrate into tissue inside the block to make the dehydration, the organization If fully dehydrated at low concentrations of alcohol, in the high concentration of alcohol in the water just off the low concentration to high concentration between Zhengzhou and therefore tissue sections can be completed in just a short time, if the time is not shortened pure alcohol time, it will cause tissue brittle; if dehydration heating, acetone, can cause tissue contraction and immunohistochemical markers affect tissue sections Quote | tissue sections | voyage teaching (view) from Xinxiang voyage teaching. Equipment Limited. "Bio-chips, teaching equipment, specimens" preferred Xinxiang voyage Teaching Equipment Co. (2), the company is located: Hongqi District Xinxiang City, Henan Province, Gong Village town freemason 207 years, teaching voyage to provide customers with the best quality Services, Contact: Manager Ma. Welcome to the new and old customers call, letter, visit the guide, business negotiations. Voyage teaching expect to be your long-term partner!