Temporary and permanent piece of sheet material sometimes sliced ??for permanent preservation of, or inability to permanently preserve the tissue sections offer, then known as the temporary sheet. Making use of a temporary piece of paraffin tissue sections reason, probably because the material can not be permanently stored, or to observe convenience only way to deal with simple materials. Further chemical agents may be used in tissue sections Henan easily change, can not be formed into a permanent piece. Another reason is the need to make a temporary piece Zuohuo body observed by the tissue or cells can remain alive for a short time to slide into. The activity tests such as pollen producers. Permanent piece of evidence is the remaining samples (slides), as proof study. Almost points based Cai Shuhua, a program for the charge material. Fixed material. Cut into thin slices. Staining. Mounted. ① ethanol dehydration dehydration levels above time can be adjusted according to the size of the organization blocks. For small tissue blocks (such as the gastric mucosa, bronchial mucosa, etc.) may be appropriate to reduce the drying time, for large tissue mass can be increased drying time, but do not exceed the maximum levels of ethanol 12h, ethanol should not exceed 4h. If unable to continue in the middle, you can return the specimen to save 80% ethanol. Further tissue slices, adipose tissue and loose connective tissue in general should be extended the dehydration time, in 95% ethanol, water must be off the net, the fat must be dissolved away (ethanol concentration of 50% or more fat soluble and lipids). Otherwise, paraffin can not penetrate the fat cells and fibrous tissue, can not cut out the good slice, but also easy to off-chip staining. ② If the tissue contained too much water (such as embryonic tissue), the dehydration of ethanol concentration should be lower-level (eg 30-40% ethanol) began, which is to ensure the production quality is very important. ③ If dehydration endless, sliced ??wax block section tissue often appears depressed (because of wax-embedded tissue blocks in contact with air and drying shrinkage). Such a wax block, though barely able to cut out sections, but the organization is also easy to fall off the effect of poor dyeing, dyeing. Therefore, you must complete dehydration, dehydration or not is completely good or bad quality of an important part of the producer. ④ volume dehydrating agent should be tissue blocks 5-10 times the total volume of dehydrating agents should be filtered and replaced. Tissue sections of tissue sections _ _ voyage supply teaching (certified merchants) provided by Xinxiang City Teaching Equipment Co. voyage. Xinxiang City voyage Teaching Equipment Co. (www.xxyuanhang.cn) located in the village of Hongqi District Xinxiang City of Henan Province town freemason 207 number. Scraps and development in the wave of market economy, the current voyage teaching has a high profile in the sample model, enjoys a good reputation. Teaching voyage made the whole network business alliance certification, marking our service and management level has reached a new height. Voyage teaching staff willing to work with all people of insight and common development, for a better future.